Regulatory

Part:BBa_K105028:Design

Designed by: Michael Wild   Group: iGEM08_ESBS-Strasbourg   (2008-10-26)

cyc70 minimal promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!

Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.



Source

Amplification and Mutation from the plasmid pAH3

References

Gudrun Bjornsdottir & Lawrence C. Myers (2008) Nucleic Acids Research 9 2906-2916.